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"Being wrong": Critical review of biodynamic neutron scattering publications

by Priv. Doc. Dr. Wolfgang Doster
retired from TU M√ľnchen and FRM 2 Munich,
an independent Web site devoted to students in biophysics applying neutron scattering techniques

Neutron Scattering is a large scale technique requiring big machines, accelerators or reactors such as the FRM2 in Garching frm.
It is freely available to the scientific public via a referee system.
Quasi-elastic neutron scattering of biomolecules reflects the time-resolved displacements of mainly hydrogen atoms
on a nano- to pico-second time scale, and is often compared with computer simulations of proteins, complementing other techniques like NMR.
The dynamic information is derived from the broadening of the spectrum in excess of the spectral resolution.
Transformed to the time domain, this yields the density correlation function versus time and momentum exchange Q,
combining the spatial and dynamic information of hydrogen displacements in the biomolecule as shown below (Doster, PNAS 2019).
The figure displays in sequence on a log time scale fast collective protein vibrations of hydrated myoglobin, side chain rotational motions
and water-coupled diffusional residue displacements.

I focus on problems, errors and questionable assumptions, which are rarely discussed in the literature.
To illustrate my point, I comment on selected neutron scattering publications in historical order.
I emphasize, that this Web site reflects my personal view, which could be wrong, unjust or incomplete.


2022: "Nanosecond structural dynamics of intrisically disordered beta-casein micelles by neutron spectroscopy"

by Nakagawa et al. pdf

2021: "Dynamics of a natively disordered protein in solution and protein force constants"
by Nakagawa et al., Biophys. J. 120, 5408 (2021) Summary

2019: Franck-Condon picture of incoherent neutron scattering
by G. Kneller, PNAS 115, 94509455 (2018), attempt to justify energy landscape models of proteins with quantum theoretical arguments.
Doster, PNAS Letter Apr. 2019,
G. Keller, Response to PNAS Letter Apr. 2019,

2018: Determination of dynamical heterogeneity of proteins from dynamic neutron scattering
Vural, Hong, Smith in Biophys. J. 114, 2397(2018)
Comment Doster
related publication by Doster Are proteins dynamically heterogeneous?

2017: Low temperature decoupling of protein and water dynamics measured by neutron scattering by A. Benedetto, Physical Chemistry Letters (2017)
Comment W. Doster, July 2019

2017: The role of momentum transfer during incoherent neutron scattering is explained by the energy landscape model
by H. Frauenfelder, R.D. Young, P.W. Fenimore PNAS vol 114, 5130(2017).
Doster Comment: the Frauenfelder zero Q elastic scattering effect reflects multiple scattering
and not the energy landscape

2015: Motional Displacement in Proteins, origin of wavevector-dependent values
by D. Vural, L. Hong, J. Smith, H. R. Glyde, Phys.Rev. E. 91 052705(2015).

Comment Doster

2015: Influence of Pressure and Crowding on the Subnanosecond Dynamics of Globular Proteins,
by M. Erlkamp, J. Marion, N. Martinez, C. Czeslik, J. Peters and R. Winter J. Phys. Chem B 119 4842(2015). Comment Doster

2014: Wave mechanical model of incoherent quasielastic neutron scattering in complex systems
by Hans Frauenfelder, Paul Fenimore and Robert Young, PNAS , 111, 12764 (2014).

Wuttke: No case against scattering theory, PNAS Letter
Doster Comment

2014: Does a dry protein undergo a glass transition by A. Frontzek, S. Strokov, ,J. Embs and S. Lushnikov, J. Phys. Chem B 118(11) 2791-2802(2014). Comment
(The Freeze Drying Glass Transition in Dry Proteins)

2013: Dynamics and Free Energy Landscape of Proteins, explored with the Mössbauer effect
and quasi-elastic neutron scattering
by Frauenfelder, Young and Fenimore, J. Phys. Chem. 117 13301 (2013)
(The Mössbauer Model of Quasi-elastic Neutron Scattering) Comment Doster

2012/2013: Change of caged dynamics of hydrated proteins by Capaccioli, Ngai, Ancherbak, Paciaroni, J. Chem.Phys. 138 (2013) 235102.
Comment Doster
Two step scenario of the protein dynamical transition
Evidence of coexistence of change of caged dynamics.. by Capaccioli, Ngai, Paciaroni, J.Phys. Chem. B 116 (2012) 1745.
submitted open Comment by W. Doster, rejected by Editor of JPCB

2011: The RENS puzzle

Elastic incoherent neutron scattering operating by varying instrumental energy resolution: Principle, simulations,
and experiments of the resolution elastic neutron scattering (RENS) S. Magazu, F. Migliardo, A. Benedetto Review of Scientific Instruments 82 (10), 105115 (2011)
nearly identical: Magazu, Migliardo, Benedetto, Vertessy in Chemical Physics 424(2013)26: Protein dynamics and neutron scattering..
Comment Doster
Comment Wuttke, Rev. Sci. Instr. 2011

2011: Protein dynamical transition at 110 K, by C. Kim, M. Tate and S. Gruner PNAS 108, 20897 (2011) Comment

2011: The Frauenfelder Mössbauer effect and the PDT
Mössbauer effect in proteins, Young, Frauenfelder, Fenimore, PRL(2011)107, 158102
Comment Doster

2008: Elliptical protein phase diagrams
Pressure and temperature dependent protein stability by Widersich, Skerra, Köhler, Friedrich, PNAS 105, 575 (2008)
Comment Doster

2006: Instrumental resolution effects interpreted as a fragile-strong crossover
Observation of fragile to strong dynamic cross-over of protein hydration water by S.H. Chen, L.Liu, E. Fratini, P. Bagliaoni, A. Faraone and E. Mamontov, PNAS USA 103, 9012 (2006)
Comment Doster

2004: Frauenfelders alpha/beta relaxation
Bulk solvent and hydration shell fluctuations by Fenimore, Frauenfelder, Mc Mahon, Young PNAS USA (2004)101,14408
Comment Doster

2003: Neutron Hydrogen Displacement Distribution in Myoglobin
Hydrogen atoms in proteins, Engler, Ostermann, Nijmura, Parak, PNAS USA (2003)100,10243
Comment Doster

2002: Slaving II
Solvent fluctuations dominate protein dynamics and function
by Fenimore, Frauenfelder, Mc Mahon, Parak, PNAS USA (2002)99,16047
Comment Doster

2002: Confined water and the two simple explanation
A model for water motion in crystals of lysozyme based on an
incoherent quasi-elastic neutron scattering study by C.Bon, A.J. Dianoux, M. Ferrand and M.S. Lehmann, Biophys. J. 83( 2002) 1578
Comment Doster

2002: The protein dynamical transition may have a simple explanantion
by M. R. Daniel, J. Finney and J. Smith, Faraday Discussion (2002) 122,163
Comment Doster

2000: Protein force constants from elastic displacements?
How soft is a protein? A protein dynamics force constant measured by neutron scattering by J. Zaccai, Science 288,1604( 2000)
Comment Doster

1998: Dynamic labelling of different functional parts of BR
by V. Reat, H. Patzelt, M. Ferrand, C. Pfister, D. Oesterhelt, G. Zaccai PNAS 95(1998)4970
Comment Doster

1998: Activity below the dynamic transition?
Enzyme Activity below the Protein Dynamical Transition at 220K by R. Daniel, J.Smith, M. Ferrand, S. Hery, R. Dunn, J. Finney, Biophys. J. 75 (1998) 2504
Comment Doster

1993: Melting of a frozen protein solution
Thermal motion and function of bacteriorhodopsin in purple membrane, effect of temperature and
hydration observed by neutron scattering by M. Ferrand, A. Dianoux, W. Petry an G. Zaccai,
PNAS 90, 9668 (1993)communicated by Hans Frauenfelder.
Comment Doster

1992: Confined water (I):
Single particle dynamics of hydration water in protein, M.C. Bellissent-Funel, J. Teixera, J.F. Bradley, S.H. Chen and L. Crespi, Physica B 181 &181, 740 (1992).

1991: Review Article on MD Simulation and Experiments
Protein Dynamics: comparison of simulations with inelastic neutron scattering experiments, by J. Smith, Quat. Rev. Biophys.24 (1991), 227
Comment Doster

1991: Frauenfelders Energy Landscapes
The energy landscapes and motions in proteins, H. Frauenfelder, S. Sligar and P. Wolynes, Science 254 (1991) 1598

1990: Vacuum simulation of a hydrated protein
Dynamics of myoglobin: comparison of simulation results with neutron scattering spectra, by J. Smith, K. Kuczera and M. Karplus, PNAS USA (1990)87, 1601.
Comment Doster

1990: First simulation of a hydrated protein: The temperature dependence of dynamics of hydrated myoglobin, comparison of force field calculations with neutron scattering data
by R. Loncharich and B. Brooks, J. Mol. Biol. (1990)215, 439

1989: first spectral analysis of protein dynamics:
Dynamical transition of myoglobin revealed by inelastic neutron scattering, W. Doster, W. Petry and S. Cusack, Nature 337,754(1989)
Comment Doster

Internal dynamics of globular proteins, comparison of neutron scattering measurements and theoretical models
by J. Smith, K. Kuczera, B. Tidor, W. Doster, S.Cusack and M. Karplus, Physica B(1989) 156, 437.
Comment Doster

1982: Ligand Binding to Hexokinase
Inelastic neutron scattering analysis of hexokinase dynamics and its modification on binding of
glucose by B. Jacrot, S. Cusack, A. Dianoux and D. Engelman, Nature 300 (1982)84
Comment Doster

1980/1996: spurious oscillations of hydration water jump rate
Molecular dynamics of hydrated proteins, H. Middendorf, J. Randall and A. J. Leadbetter, Phil. Trans. R. Soc. Lond.B. (1980) 290, 639. and Middendorf, Phys. B. 226, 113 (1996)
Comment Doster

last changes: May 5th, 2022